Factors affecting in vitro propagation ofYucca glauca

✍ Scribed by Susan E. Bentz; Bruce J. Parliman; Helen-Jean Talbott; William L. Ackerman

• Book ID: 104619128
• Publisher: Springer
• Year: 1988
• Tongue: English
• Weight: 480 KB
• Volume: 14
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00041184

✦ Synopsis

A micropropagation system was developed to facilitate the release and subsequent testing of unique pink-or white-flowered selections of Yucca glauca. Shoot tip explants from mature plants were cultured on Murashige and Skoog medium supplemented with factorial combinations of ~t-naphthaleneacetic acid (NAA) (0.0 to 3.2#M) and 6-benzylaminopurine (BA) (0.0 to 45/~M). Shoots were found to proliferate with increasing concentrations of BA and to produce callus and poorer quality shoots in the presence of NAA and the absence of BA. The response to BA and NAA was similar among 3 genotypes examined. A comparison of BA and N6-(A 2-isOpentenyladenine) (2iP) showed that 2iP was not effective in promoting shoot proliferation. Shoot tips rooted in the absence of growth regulators or in the presence of low concentrations of indole-3-butyric acid (I BA). Plantlets were successfully acclimated to greenhouse conditions.