Factors affecting hepatocyte isolation, engraftment, and replication in an in vivo model

✍ Scribed by Ricky H. Bhogal; Simon C. Afford

• Book ID: 102468663
• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 41 KB
• Volume: 16
• Category: Article
• ISSN: 1527-6465
• DOI: 10.1002/lt.22177

✦ Synopsis

We congratulate Kawahara et al. 1 on their excellent study published in Liver Transplantation. We read about their experiences with human hepatocyte isolation and the subsequent application of the isolated cells with keen interest. Our group also has good experience with human hepatocyte isolation. 2 We have 2 points to raise with respect to this excellent study. The authors used human hepatocytes isolated from resected normal liver tissue in their study (51 cancer specimens and 39 specimens of benign etiology). Our data show that such hepatocytes differ in their response to hypoxia and hypoxia/reoxygenation in comparison with hepatocytes isolated from normal livers. 2 Did the authors note any phenotypic differences between hepatocytes isolated from cancer specimens and those isolated from benign livers? Moreover, did this have a bearing on transplant success? Kawahara et al. 1 stated that their initial experiences with the isolation of human hepatocytes from cirrhotic livers was poor in terms of viable cell yields and transplant success. In our laboratory, we isolate human hepatocytes from all explanted livers after transplantation (for primary biliary cirrhosis, primary sclerosing cholangitis, alcoholic liver cirrhosis, or metabolic liver disease), from donor livers exceeding surgical requirements, and from normal tissue obtained from hepatic resections (for metastatic disease). We do not isolate the hepatocytes of patients with a viral hepatic disease. Although we have not published our results yet, we can state that our overall success rate for attempted human hepatocyte isolation is 54%.