Factors affecting flow cytometric detection of apoptotic nuclei by DNA analysis
β Scribed by Kenneth H. Elstein; David J. Thomas; Robert M. Zucker
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 625 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
β¦ Synopsis
E 1995 WileyLiss, Inc as such. i5 in thc public domain in the United States of America.
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A new modification of our detergent technique for the preparation of nuclei for flow cytometric DNA analysis is described. The attainment of low coefficients of variation of the peaks and of quantitative staining of nuclei from different tissues was a problem with the original method. This was solve
## Abstract We describe an algorithm for fully automated flow cytometric DNA histogram classification and analysis that provides rapid, reproducible determination of DNA index and Sβphase fraction (SPF). Automated classification agreed with subjective assessment of DNA ploidy in 96β98% of DNA histo