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Factors affecting ectopic gene conversion in mice

✍ Scribed by Deoborah M. Cooper; Kerry J. Schimenti; John C. Schimenti

Publisher
Springer-Verlag
Year
1998
Tongue
English
Weight
899 KB
Volume
9
Category
Article
ISSN
0938-8990

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Electroporation was used to evaluate parameters affecting transient gene expression in Glycine max protoplasts. Protoplast viability and reporter enzyme activity for chloramphenicol acetyl transferase (CAT) and ß-glucuronidase (GUS) depended on the field strength employed. Maximum CAT and GUS activi