Facilitation of Ca2+Action Potential Frequency by a Small G Protein Rab3A in Rat Pituitary GH3Cells
โ Scribed by Nobuyuki Suzuki; Moritoshi Hirono; Hiroshi Takagi; Tohru Yoshioka
- Book ID
- 115580389
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 133 KB
- Volume
- 235
- Category
- Article
- ISSN
- 0006-291X
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## Abstract **Background:** Rab3A, a member of the Rab3 small G protein family, regulates Ca^2+^โdependent exocytosis of neurotransmitter. The cyclical activation and inactivation of Rab3A are essential for the Rab3A action in exocytosis. GDPโRab3A is activated to GTPโRab3A by Rab3 GDP/GTP exchange
## Abstract The effects of rottlerin, a known inhibitor of protein kinase Cโฮด activation, on ion currents were investigated in pituitary tumor (GH~3~) cells. Rottlerin (0.3โ100 ยตM) increased the amplitude of Ca^2+^โactivated K^+^ current (I~K(Ca)~) in a concentrationโdependent manner with an EC~50~