Facilitating the pickup of individual DNA molecules by AFM nanomanipulation with tips mechanically worn on bare mica
✍ Scribed by Na Duan; Fei Long; Xinyan Wang; Bin Li; Jun Hu; Yi Zhang
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 232 KB
- Volume
- 75
- Category
- Article
- ISSN
- 1059-910X
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✦ Synopsis
Abstract
The tip is one of the critical factors to improve the efficiency in picking up individual DNA molecules from solid substrates based on atomic force microscope (AFM) nanomanipulation. We found that wearing AFM tips on certain solid substrates in advance to nanomanipulation operation would largely improve the pickup efficiency, which was ascribed to the increasing affinity of the tip to the DNA molecules along with the increase of the tip radius after wearing. It was demonstrated that bare mica was superior to APTES‐modified mica to keep the tip clean while wearing, which was crucial for DNA pickup during AFM nanomanipulation. Microsc. Res. Tech., 2012. © 2011 Wiley Periodicals, Inc.