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Fabrication of Substrates with Defined Mechanical Properties and Topographical Features for the Study of Cell Migration

✍ Scribed by Jonathan M. Charest; Joseph P. Califano; Shawn P. Carey; Cynthia A. Reinhart-King

Publisher: John Wiley and Sons
Year: 2011
Tongue: English
Weight: 620 KB
Volume: 12
Category: Article
ISSN: 1616-5187
DOI: 10.1002/mabi.201100264

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Both substrate topography and substrate mechanical properties are known to influence cell behavior, but little is known about how they act in concert. Here, a method is presented to introduce topographical features into PA hydrogel substrates that span a wide range of physiological E values. Gel swelling plays a significant role in the fidelity of protruding micromolded features, with the most efficient pattern transfer occurring at a crosslinking concentration equal to or greater than ≈5%. In contrast, swelling does not influence the spacing fidelity of microcontact printed islands of collagen on 2D PA substrates. BAECs cultured on micromolded PA substrates exhibit contact guidance along ridges patterned for all E tested.magnified image

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