Fabrication of PDMS/glass microchips by twofold replication of PDMS and its application in genetic analysis
✍ Scribed by Xiaofeng Qu; Fuming Sang; Jicun Ren
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 741 KB
- Volume
- 29
- Category
- Article
- ISSN
- 1615-9306
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✦ Synopsis
Abstract
In this paper, we describe a simple method for fabrication of high quality poly(dimethylsiloxane) (PDMS)/glass microchip by twofold replica molding of PDMS. This technique first served to transfer the negative microchannels from the glass template to the PDMS substrate as a master, and then this PDMS master with positive microchannels was used to replicate the PDMS replica with negative microchannels. Finally, the PDMS replica was bound to a glass sheet by UV radiation. The fabricated microchips were successfully applied for the detection of C677T mutation from the human methylenetetrahydrofolate reductase gene.