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Fabrication of nano-structured diamond films for SAOS-2 cell cultivation

✍ Scribed by Babchenko, Oleg ;Kromka, Alexander ;Hruska, Karel ;Kalbacova, Marie ;Broz, Antonin ;Vanecek, Milan

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
709 KB
Volume
206
Category
Article
ISSN
0031-8965

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✦ Synopsis

Abstract

The present work reports on application of gold, nickel, and diamond nano‐particles as a masking material for realization of diamond 3D nano‐structures after a low power (100 W) plasma etching process in a gas mixture of CF~4~/O~2~. Obtained 3D structures strongly depend on the masking material. Application of the Ni mask results in diamond nano‐rods, whereas the Au mask leads to homogenous “ultra” nano‐cone structures. The diamond powder application results in irregular nano‐cone structures. The present procedure uses a strategy where no lithographic steps are implemented. Prepared samples are tested as the substrates for human osteoblast‐like cells cultivation. Obtained results exhibit a distinctive behavior in cell adhesion on used surface morphologies, as demonstrated by immunofluorescent staining of vinculin (a structural protein of focal adhesion).

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