Fabrication of nano-hydroxyapatite on electrospun silk fibroin nanofiber and their effects in osteoblastic behavior

✍ Scribed by Kai Wei; Yuan Li; Kyu-Oh Kim; Yuya Nakagawa; Byoung-Suhk Kim; Koji Abe; Guo-Qiang Chen; Ick-Soo Kim

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 766 KB
• Volume: 97A
• Category: Article
• ISSN: 1549-3296
• DOI: 10.1002/jbm.a.33054

✦ Synopsis

Abstract

In this study, a novel tissue engineering scaffold material of electrospun silk fibroin/nano‐hydroxyapatite (nHA) biocomposite was prepared by means of an effective calcium and phosphate (Ca‐P) alternate soaking method. nHA was successfully produced on regenerated silk fibroin nanofiber as a substrate within several minutes without any pretreatments. The morphologies of both nonmineralized and mineralized nanofibers were analyzed using a field‐emission scanning electron microscopy (FESEM). The crystallographic phases of the nHA were analyzed using X‐ray diffraction (XRD). Fourier transform infrared (FTIR) spectrophotometer and thermogravimetry analyses (TGA) were employed to determine the type of functional groups and the amount of nHA presenting in the silk/nHA biocomposite nanofibers, respectively. The osteoblastic activities of this novel nanofibrous biocomposite scaffold were also investigated by employing osteoblastic‐like MC3T3‐E1 cell line. The cell functionality such as alkaline phosphatase (ALP) activity was ameliorated on mineralized nanofibers. All these results indicated that this silk/nHA biocomposite scaffold material may be a promising biomaterial for bone tissue engineering. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 2011.