Fabrication of DNA Polymer Brush Arrays by Destructive Micropatterning and Rolling-Circle Amplification
✍ Scribed by Kristopher D. Barbee; Matt Chandrangsu; Xiaohua Huang
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 749 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1616-5187
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
A method for fabricating DNA polymer brush arrays using photolithography and plasma etching followed by solid‐phase enzymatic DNA amplification is reported. After attaching oligonucleotide primers to the surface of a glass coverslip, a thin layer of photoresist is spin‐coated on the glass and patterned via photolithography to generate an array of posts in the resist. An oxygen‐based plasma is then used to destroy the exposed oligonucleotide primers. The glass coverslip with the primer array is assembled into a microfluidic chip and DNA polymer brushes are synthesized on the oligonucleotide array by rolling‐circle DNA amplification. We have demonstrated that the linear polymers can be rapidly synthesized in situ with a high degree of control over their density and length. magnified image