Fabrication and characterization of a biomimetic composite scaffold for bone defect repair

✍ Scribed by H. Nitzsche; A. Lochmann; H. Metz; A. Hauser; F. Syrowatka; E. Hempel; T. Müller; T. Thurn-Albrecht; K. Mäder

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 384 KB
• Volume: 94A
• Category: Article
• ISSN: 1549-3296
• DOI: 10.1002/jbm.a.32703

✦ Synopsis

Abstract

For successful bone tissue engineering, scaffolds with tailored properties are a basic requirement. The combination of different available materials not only appears to be desirable but also very challenging. In this study, a composite material consisting of hydroxyapatite and collagen was produced by a biomimetic precipitation method and characterized by X‐ray diffraction (XRD) and thermogravimetry (TGA). Subsequently, a suspension‐quick‐freezing and lyophilization method was used to incorporate the hydroxyapatite into a polymeric matrix consisting of collagen and chitosan. Before physicochemical characterization, the highly porous scaffolds were consolidated by a dehydrothermal treatment (DHT). The main attention was focused on the particle size of hydroxyapatite, which should be in the nanometer range. This is relevant to achieve a homogeneous resorption of the material by osteoclasts. Small‐angle X‐ray scattering (SAXS), atomic force microscopy (AFM), and environmental scanning electron microscopy (ESEM) were used to evaluate the outcome. The results suggest a successful polymeric embedding of nanoscaled hydroxyapatite particles into the matrix of the spongy construct. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010