Extraordinary high expression of transgenes in stably transfected mammalian cells
β Scribed by Corni Strydom; Wouter de Wet
- Book ID
- 117640473
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 59 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0945-053X
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π SIMILAR VOLUMES
## Abstract ## Background Supercoiled topology of transfected plasmid DNA (pDNA) is critical for transgene expression in mammalian cells. In the present study, we analysed transgene expression of transfected supercoiled pDNA concatemers. ## Methods Jurkat T cells were transfected with a supercoi
We read with interest the article by Maucksch et al. [1], who analysed transgene expression of transfected supercoiled 4.7 kb monomeric and 9.4 kb dimeric plasmid concatemer in Jurkat T cells using electroporation. The authors reported that the relative number of electrotransferred gene copies per n