Rat hepatoma cells (Fu5AHj were studied as a model for the net delivery of apoE-free high-density lipoprotein (HDL) cholesterol to a cell. Incubating cells with HDL results in 1) a decrease in both media-free cholesterol and cholesteryl ester concentration; 2) decreased cell sterol synthesis; and 3)
Extralysosomal degradation of high-density lipoproteins in a human hepatoma cell line, Mahlavu
โ Scribed by Sakai Naohiko; Yamashita Shizuya; Ueyama Yuhya; Kawamoto Toshiharu; Nakamura Tadashi; Funahashi Tohru; Kameda-Takemura Kaoru; Kawata Sumio; Kubo Masaharu; Tokunaga Katsuto; Tarui Seiichiro; Matsuzawa Yuji
- Book ID
- 115741161
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 748 KB
- Volume
- 1169
- Category
- Article
- ISSN
- 0005-2760
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Low density lipoprotein (LDL) processing was investigated in a human hepatoma-derived cell line, Hep G2. Hep G2 cells bound, internalized and degraded LDL via a saturable, high affinity (Kd -2 x lo-' M ) pathway similar to that present in other mammalian cells. Although 80% of the uptake and degrada