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Extraction of clavulanic acid using aqueous two-phase micellar system

✍ Scribed by Evelyn Guedes de Andrade; Marcela de Siqueira Cardoso Silva; Raquel Brandão Haga; Valéria Carvalho Santos; Adalberto Pessoa Jr.; Carlota de O. Rangel-Yagui

Publisher
Portland Press
Year
2011
Tongue
English
Weight
241 KB
Volume
58
Category
Article
ISSN
0885-4513

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✦ Synopsis

An investigation of clavulanic acid behavior in an aqueous two-phase micellar system employing the surfactants n-decyltetraethylene oxide (C 10 E 4 ) and dodecyldimethylamine oxide (DDAO) was carried out. According to the results, clavulanic acid partitions evenly between the two phases of DDAO micellar system, mixed DDAO-C 10 E 4 micellar system, as well as C 10 E 4 micellar system. Therefore, electrostatic interactions between positively charged DDAO-containing micelles and negatively charged drug were not strong enough to influence the partitioning. Nevertheless, clavulanic acid extraction from Streptomyces clavuligerus fermentation broth in C 10 E 4 micellar system employing a previous protein denaturation step provided recovery of 52% clavulanic acid with removal of 70% of the contaminant proteins, which is already promising as a purification strategy.

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