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Extraction and quantitation of total cholesterol, dolichol and dolichyl phosphate from mammalian liver

✍ Scribed by Dean C. Crick; Kenneth K. Carroll

Book ID
112774353
Publisher
Springer-Verlag
Year
1987
Tongue
English
Weight
393 KB
Volume
22
Category
Article
ISSN
0024-4201

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A procedure for the quantitative extraction of both dolichol and dolichyl phosphate (Dol-P) in plant tissue (soybean embryos) into diethyl ether from an alkaline saponification mixture is described. A complete and quantitative separation of total dolichol and total Dol-P is then obtained based on th