Extraction and purification of amyloglucosidase produced by solid state fermentation withAspergillus niger

✍ Scribed by Mangaleswary Ramadas; Olle Holst; Bo Mattiasson

• Publisher: Springer-Verlag
• Year: 1995
• Tongue: English
• Weight: 549 KB
• Volume: 9
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00158544

✦ Synopsis

Aqueous two-phase extraction was employed, as a new approach, for the recovery of amyloglucosidase (AMG) after solid state fermentation. In an aqueous two-phase system of PEG 6000 and potassium phosphate, 95% of the AMG was recovered in the bottom salt phase, with all visible particles from solid fungal bran in the interface, giving a purification factor of 11. Affinity purification of AMG was also done by adsorption onto crosslinked starch, in the presence of PEG or salt. Both were found to enhance the adsorption. In the presence of PEG 6000 (10% w/w), two fold increase in AMG adsorption, 85% of AMG recovery and 6.0 fold of purification were achieved. The purified AMG was found homogeneous by SDS polyacrylamide gel electrophoresis.