Extraction and analysis of superoxide free radicals (·O−2) from whole mammalian liver

✍ Scribed by Shoaf, Antony R. ;Shaikh, Ali U. ;Harbison, Raymond D. ;Hinojosa, Oscar

• Book ID: 102762058
• Publisher: John Wiley and Sons
• Year: 1991
• Weight: 680 KB
• Volume: 6
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170060206

✦ Synopsis

Extraction o f whole lobes o f normal rat liver with dimethyl sulphoxide (DMSO) under N, gives extracts which contain ClOprnol/l-O; (50-100 nmol-0; per 10 m l extract per 4 g liver; 1.25-2.50 nmol -0; per millilitre per gram liver). Evidence for -0; in the extracts is given by: (1) electron spin resonance signals (ESR), (2) differential pulse polarography (DPP), ( 3) chemiluminescence (CL), and (4) nitroblue tetrazolium reduction (NBT). All tests yield results identical with those obtained w i t h authentic -0 ; . Extraction o f -0; is enhanced by tetrabutyl ammonium ion, and is maximal at 1-3 min. These results raise the possibility that substantial amounts o f ~0 ; are normally sequestered in protective membranous sites in vivo.