✦ LIBER ✦

Extracting and visualizing matrix-assisted laser desorption/ionization time-of-flight mass spectral fingerprints

✍ Scribed by Kristin H. Jarman; Don S. Daly; Catherine E. Petersen; Adam J. Saenz; Nancy B. Valentine; Karen L. Wahl

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 163 KB
Volume: 13
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(19990815)13:15<1586::aid-rcm680>3.0.co;2-2

No coin nor oath required. For personal study only.

✦ Synopsis

We have developed a method for constructing and extracting matrix-assisted laser desorption/ionization (MALDI) fingerprints. This method is fully automated and statistically based, allowing a large number of spectra to be analyzed at a time in an objective manner. This method can be used to extract the fingerprint of a particular analyte from a spectrum containing multiple analytes. Therefore, this method lends itself well to real-world applications where samples to be analyzed are likely to be impure. We illustrate this method on experimental results from a series of studies of E. coli and B. atrophaeus MALDI time-of-flight mass spectrometry (TOFMS) fingerprints.

📜 SIMILAR VOLUMES

Precalibration of matrix-assisted laser

Precalibration of matrix-assisted laser desorption/ionization-time of flight spectra for peptide mass fingerprinting

✍ Assaf Wool; Zeev Smilansky 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 107 KB

Identification of proteins using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) peptide mass fingerprinting (PMF) is a key technique in proteomics. The method is known to be sensitive as well as amenable to high-throughput operation, but the resulting identifications suffer f

Ionization mechanism of oligonucleotides

Ionization mechanism of oligonucleotides in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

✍ Yufei Kong; Yifei Zhu; Jing-Yuan Zhang 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 171 KB 👁 2 views

The ionization of nucleosides in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was systematically investigated using adenine (A), thymine (T), guanine (G) and cytosine (C) with several common matrices. Experimental results of the protonation and deprotonation of the ba

High-resolution infrared laser desorptio

High-resolution infrared laser desorption/ionization and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of synthetic polymers

✍ Cramer, Rainer; Burlingame, Alma L. 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 77 KB 👁 2 views

High-resolution Infrared Laser Desorption/Ionization and Matrix-assisted

Time-of-flight effects in matrix-assiste

Time-of-flight effects in matrix-assisted laser desorption/ionization fourier transform mass spectrometry

✍ T.-P. E. Sze; T.-W. Dominic Chan 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 137 KB 👁 2 views

The time-of-flight effect in Fourier transform mass spectrometry (FTMS) with an external ion source was quantified. The time-of-flight profiles of ions of different masses were measured by stepping the time interval between the ionization pulse and the gating of the trapping voltage (T gate ). The o

Matrix-assisted laser desorption/ionizat

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of kerogen extracts: effect of interactions between sample structure and matrix

✍ A. A. Herod; M.-J. Lazaro; M. Rahman; M. Domin; M. J. Cocksedge; R. Kandiyoti 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 194 KB 👁 1 views

This paper describes experiments carried out within the framework of examining capabilities of MALDI-TOF-MS in the characterisation of fossil fuels and related materials. The work has focused on the effect of interactions between sample structure and matrix composition on the quality of MALDI spectr

Matrix-assisted laser desorption/ionizat

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of canola, castor and olive oils

✍ Folahan O. Ayorinde; Eisa Elhilo; Caswell Hlongwane 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 38 KB 👁 1 views

A fast-drying sample preparation method, using a-cyano-4-hydroxycinnamic acid as a matrix in an acetonitrile/tetrahydrofuran solvent system, has been used to obtain the matrix-assisted laser desorption/ ionization (MALDI) time-of-flight (TOF) mass spectra of commercial canola, castor and olive oils.