Extrachromosomal gene amplification in acute myeloid leukemia; Characterization by metaphase analysis, comparative genomic hybridization, and semi-quantitative PCR
✍ Scribed by A. N. Mohamed; J. A. Macoska; A. Kallioniemi; O.-P. Kallioniemi; F. Waldman; V. Ratanatharathorn; S. R. Wolman
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 440 KB
- Volume
- 8
- Category
- Article
- ISSN
- 1045-2257
No coin nor oath required. For personal study only.
✦ Synopsis
A case of acute myeioid leukemia (M-3) with complex karyotypic aberrations and double minute (dmin) chromosomes is presented. The patient had no history of prior exposure to mutagenic or carcinogenic agents or of other malignancies. She died from CNS involvement six weeks after the initial diagnosis. We used comparative genomic hybridization to identify the amplified sequences presumed to represent the dmin of the leukemic cells; the turnorlnormal ratios indicated increased signal intensity at 8q24. This localization prompted investigation by semi-quantitative PCR that revealed amplification of the MYC oncogene. The extent of chromosome aberrations and the oncogene amplification, both linked with poor prognosis, may relate to the rapid course of this patient's disease.