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Extracellular production of active vibriolysin engineered by random mutagenesis in Escherichia coli

✍ Scribed by Hiroyuki Sonoda; Atsushi Sugimura

Book ID
116828873
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
418 KB
Volume
62
Category
Article
ISSN
1046-5928

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## Abstract The extracellular production of penicillin acylase (PAC) in genetically engineered __Escherichia coli__ by coexpression of the __brp__ gene encoding bacteriocin release protein (BRP) and the __pac__ gene was demonstrated. Cell physiology was affected while PAC was released into the medi