✦ LIBER ✦

Extracellular Matrix Functionalized Microcavities to Control Hematopoietic Stem and Progenitor Cell Fate

✍ Scribed by Ina Kurth; Katja Franke; Tilo Pompe; Martin Bornhäuser; Carsten Werner

Publisher: John Wiley and Sons
Year: 2011
Tongue: English
Weight: 706 KB
Volume: 11
Category: Article
ISSN: 1616-5187
DOI: 10.1002/mabi.201000432

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Polymeric microcavities functionalized with extracellular matrix components were used as an experimental in vitro model to investigate principles of hematopoietic stem and progenitor cell (HSPC) fate control. Using human CD133+ HSPC we could demonstrate distinct differences in HSPC cycling and differentiation dependence on the adhesion ligand specificity (i.e., heparin, collagen I) and cytokine levels. The presented microcavity platform provides a powerful in vitro approach to explore the role of exogenous cues in HSPC fate decisions and can therefore be instrumental to progress in stem cell biology and translational research toward new therapies. magnified image

📜 SIMILAR VOLUMES

Impact of maturational status on the abi

Impact of maturational status on the ability of osteoblasts to enhance the hematopoietic function of stem and progenitor cells

✍ Ying-Hua Cheng; Brahmananda R Chitteti; Drew A Streicher; Joseph A Morgan; Sonia 📂 Article 📅 2011 🏛 American Society for Bone and Mineral Research 🌐 English ⚖ 346 KB 👁 1 views

Osteoblasts (OBs) exert a prominent regulatory effect on hematopoietic stem cells (HSCs). We evaluated the difference in hematopoietic expansion and function in response to co-culture with OBs at various stages of development. Murine calvarial OBs were seeded directly (fresh) or cultured for 1, 2, o