Extracellular matrix components produced by SV40-transformed human epidermal keratinocytes

✍ Scribed by Keith W. Brown; E. Kenneth Parkinson

• Book ID: 102864910
• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: French
• Weight: 847 KB
• Volume: 33
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910330215

✦ Synopsis

The production of fibronectin, laminin and glycosaminoglycans (GAGs) has been investigated in normal and SV40-transformed human epidermal keratinocytes. Normal keratinocytes pruduced small amounts of laminln and fibronectin and three classes of GAGs; hyaluronic acid, heparan sulphate and chondroitin sulphate, hyaluronic acid being the major GAG. The SV4OO-transformed keratinocytes retained their cell-surface laminin and fibronectin, and in some cases showed elevated levels when compared to normal keratinocytes. All the transformants showed a shift in GAG production from hyaluronic acid to heparan sulphate, so that in all SV40-transformed keratinocytes heparan sulphate was the major GAG. These results demonstrate that the loss of extracellular matrix, which normally occurs in transformed fibroblastic cells, is not a feature of SV40-transformed human keratinocytes.