Extracellular deamination ofl-amino acids byChlamydomonas reinhardtiicells
✍ Scribed by J. Muñoz-Blanco; J. Hidalgo-Martínez; J. Cárdenas
- Book ID
- 104659515
- Publisher
- Springer-Verlag
- Year
- 1990
- Tongue
- English
- Weight
- 423 KB
- Volume
- 182
- Category
- Article
- ISSN
- 0032-0935
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✦ Synopsis
When grown in the light and in a Tris-acetate phosphate medium, cells of Chlamydomonas reinhardtii Dang. can use the following L-amino acids as a sole nitrogen source: asparagine, glutamine, arginine, lysine, alanine, valine, leucine, isoleucine, serine, methionine, histidine, and phenylalanine, whereas, in the absence of acetate, the cells only used L-arginine. The utilization system in the acetate medium consisted of an extracellular deaminating activity induced by L-amino acids; it took between 10 to 30 h before the system appeared in cells previously grown with ammonium. This deaminase activity was nonspecific, required an organic carbon source for its de-novo synthesis, and was sensitive to high ammonium concentration and light deprivation.