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Extracellular biosynthesis and characterization of silver nanoparticles using Aspergillus flavusNJP08: A mechanism perspective

✍ Scribed by Jain, Navin ;Bhargava, Arpit ;Majumdar, Sonali ;Tarafdar, J. C. ;Panwar, Jitendra

Book ID
118185546
Publisher
The Royal Society of Chemistry
Year
2011
Tongue
English
Weight
446 KB
Volume
3
Category
Article
ISSN
2040-3364

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✦ Synopsis

The present study demonstrates an eco-friendly and low cost protocol for synthesis of silver nanoparticles using the cell-free filtrate of Aspergillus flavus NJP08 when supplied with aqueous silver (Ag + ) ions. Identification of the fungal isolate was based on nuclear ribosomal DNA internal transcribed spacer (ITS) identities. Transmission electron microscopy (TEM) and energy dispersive spectroscopy (EDS) revealed the formation of spherical metallic silver nanoparticles. The average particle size calculated using Dynamic Light Scattering measurements (DLS) was found to be 17 AE 5.9 nm. UV-Visible and Fourier transform infrared (FTIR) spectroscopy confirmed the presence of extracellular proteins. SDS-PAGE profiles of the extracellular proteins showed the presence of two intense bands of 32 and 35 kDa, responsible for the synthesis and stability of silver nanoparticles, respectively. A probable mechanism behind the biosynthesis is discussed, which leads to the possibility of using the present protocol in future ''nano-factories''.

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## Abstract The development of an eco‐friendly and reliable process for the synthesis of gold nanomaterials using microorganisms is gaining importance in the field of nanotechnology. In the present study, gold nanoparticles have been synthesized by reduction of aqueous gold ions using the culture s