Starch gel electrophoresis with two different buffer systems and several substrates and inhibitors have been used to study the electrophoretic variability of esterases in leaves of cultivars of Triticum aestivum. Each one of the buffer systems showed different levels of variability, according to the
Extent of genetic variability of endosperm esterases inTriticum aestivumL. 2n=6x=42
โ Scribed by L. Rebordinos; M. Perez Vega
- Book ID
- 104689906
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 585 KB
- Volume
- 78
- Category
- Article
- ISSN
- 0040-5752
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โฆ Synopsis
Genetic variability of endosperm esterase has been studied in 42 cultivars of Triticum aestivum L. 2n=6x=42. Different techniques, including sequential electrophoresis and electrofocusing, have been used with various substrates and esterase inhibitors. The electrophoretic patterns in each cultivar are described. Chromosomal location using the nullitetrasomic and ditelosomic lines of Chinese Spring was carried out in order to relate and/or locate the esterase genes to specific chromosomes. Most of the esterase isozymes located were in the long arm of the chromosomes of the homoeology group 3; but we have found six located in the short arms, five of them in the chromosome 3AS and one in the 3DS. This location increases the number of esterase genes described, because no esterase genes had been described so far in short arms of chromosomes of the homoeology group 3. The genetic control is discussed and, according to our results, between 12 and 15 loci, organized in five "compound loci", control the endosperm esterases in wheat. Also one "modifier" gene modifying the mobility of two esterase bands and present in all the cultivars studied is postulated.
๐ SIMILAR VOLUMES
Triticum tauschii (Coss.) Schmalh. (Aegilops squarrosa auct. non L., 2n --2x = 14, DD genome) with its diverse range of accessions and distribution provides a unique opportunity for exploiting novel genetic variability for wheat (T. aestivum L.) improvement associated with biotic/abiotic stress fact