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Expression, purification, stability optimization and characterization of human Aurora B kinase domain from E. coli

✍ Scribed by Payal R. Sheth; Lata Ramanathan; Ashwin Ranchod; Andrea D. Basso; Dianah Barrett; Jia Zhao; Kimberly Gray; Yan-Hui Liu; Rumin Zhang; Hung V. Le


Book ID
116178711
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
894 KB
Volume
503
Category
Article
ISSN
0003-9861

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✍ Nicholas A. Flint; Kurt E. Amrein; Thomas Jascur; Paul Burn πŸ“‚ Article πŸ“… 1994 πŸ› John Wiley and Sons 🌐 English βš– 898 KB

The lymphocyte-specific, nonreceptor protein tyrosine kinase Lck has been purified from an Escherichia coli expression system using a monoclonal antibody column followed by dye-affinity chromatography. Polyacrylamide gel electrophoretic analysis of purified protein revealed a single 56 kDa band, ind