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Expression, purification, reconstitution and inhibition of Ustilago maydis sterol 14α-demethylase (CYP51; P45014DM)

✍ Scribed by David C Lamb; Diane E Kelly; Nigel J Manning; Derek W Hollomon; Steven L Kelly


Book ID
117518995
Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
297 KB
Volume
169
Category
Article
ISSN
0378-1097

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Human and Candida albicans CYP51 were purified to homogeneity after GAL10-based heterologous expression in yeast in order to resolve the basis for the selective inhibition of the fungal enzyme over the human orthologue by the azole drugs ketoconazole and itraconazole, used in the treatment of system