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Expression, purification, and refolding of active human and mouse secreted group IIE phospholipase A2

โœ Scribed by Yujie Zhang; Tingting Xu; Qiujia Chen; Bing Wang; Jinsong Liu

Book ID
116829335
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
554 KB
Volume
80
Category
Article
ISSN
1046-5928

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## Abstract A secreted form of phospholipase A~2~ (PLA~2~) is thought to play an important role in inflammatory diseases. To characterize this enzyme the cDNA encoding a low molecular weight PLA~2~ was cloned from a human placental cDNA library. The cDNA encoding the human PLA~2~ was subcloned into