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Expression, purification, and refolding of a novel immunotoxin containing humanized single-chain fragment variable antibody against CTLA4 and the N-terminal fragment of human perforin

✍ Scribed by Lin Wan; Lingyu Zeng; Lihong Chen; Qiang Huang; Shengfu Li; Yanrong Lu; Youping Li; Jingqiu Cheng; Xiaofeng Lu


Book ID
116828353
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
265 KB
Volume
48
Category
Article
ISSN
1046-5928

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✍ Reza M. Seifar; Robbert H. Cool; Wim J. Quax; Rainer Bischoff πŸ“‚ Article πŸ“… 2004 πŸ› John Wiley and Sons 🌐 English βš– 139 KB

## Abstract Immunoaffinity capillary electrophoresis and surface plasmon resonance have been used for the characterization of the interaction between two large‐sized proteins, the human complement protein C4 and the single‐chain variable fragment C43. The rather high kinetic rate constants as deter