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Expression, Purification, and Initial Characterization of Human Yes Protein Tyrosine Kinase from a Bacterial Expression System

โœ Scribed by Gongqin Sun; Raymond J.A. Budde


Book ID
115566637
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
192 KB
Volume
345
Category
Article
ISSN
0003-9861

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The lymphocyte-specific, nonreceptor protein tyrosine kinase Lck has been purified from an Escherichia coli expression system using a monoclonal antibody column followed by dye-affinity chromatography. Polyacrylamide gel electrophoretic analysis of purified protein revealed a single 56 kDa band, ind