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Expression, Purification and Enzymatic Characterization of the Catalytic Domains of Human Tryptophan Hydroxylase Isoforms

✍ Scribed by Michael S. Windahl; Jane Boesen; Pernille E. Karlsen; Hans E. M. Christensen

Publisher
Springer
Year
2009
Tongue
English
Weight
279 KB
Volume
28
Category
Article
ISSN
1573-4943

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## Abstract We have cloned, over expressed, and purified one of the two catalytic domains (residues Ala^361^ to Gly^468^, ACE‐N) of human somatic angiotensin‐I converting enzyme in __Escherichia coli.__ This construct represents the __N__‐catalytic domain including the two binding motifs and the 23