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Expression, Purification, and Characterization of an Iron Chaperon Protein CyaY fromAcidithiobacillus ferrooxidans

✍ Scribed by Chenbing Ai; Hongyu Mo; Qian Chen; Yuandong Liu; Lin Tang; Juan Du; Jia Zeng


Publisher
Springer
Year
2010
Tongue
English
Weight
446 KB
Volume
62
Category
Article
ISSN
0343-8651

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The lymphocyte-specific, nonreceptor protein tyrosine kinase Lck has been purified from an Escherichia coli expression system using a monoclonal antibody column followed by dye-affinity chromatography. Polyacrylamide gel electrophoretic analysis of purified protein revealed a single 56 kDa band, ind