Expression, purification, and characterization of 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase from Pyrococcus furiosus
โ Scribed by Linley R Schofield; Mark L Patchett; Emily J Parker
- Book ID
- 116827769
- Publisher
- Elsevier Science
- Year
- 2004
- Tongue
- English
- Weight
- 289 KB
- Volume
- 34
- Category
- Article
- ISSN
- 1046-5928
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The phenylalanine-regulated isozyme of 3-deoxy-D-arabino-heptulosonate-7phosphate synthase (DAHPS) from Escherichia coli, its binary complexes with either substrate, phosphoenolpyruvate (PEP), or feedback inhibitor, Phe, and its ternary complexes with either PEP or Phe plus metal cofactor (either Mn
The first step in the biosynthesis of aromatic amino acids by Candida maltosa was eatalysed by two isozymes of 3-deoxy-~-arabino-heptulosonate 7-phosphate (DAHP) synthase. The formation of which was constitutive. The phenylalanine-sensitive and the tyrosine-sensitive DAHP synthases were partially pu