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Expression, Purification, and C-terminal Site-Specific PEGylation of Cysteine-Mutated Glucagon-Like Peptide-1

โœ Scribed by Mingming Gao; Hong Tian; Chen Ma; Xiangdong Gao; Wei Guo; Wenbing Yao


Book ID
107565411
Publisher
Springer-Verlag
Year
2009
Tongue
English
Weight
257 KB
Volume
162
Category
Article
ISSN
0273-2289

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Pharmacokinetic and pharmacodynamic eval
โœ Su Young Chae; Young Goo Chun; Seulki Lee; Cheng-Hao Jin; Eun Seong Lee; Kang Ch ๐Ÿ“‚ Article ๐Ÿ“… 2009 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 337 KB

The rapid elimination of glucagon-like peptide-1 (GLP-1) is the main impediment to its anti-diabetic utility. Here, we tried to improve its poor pharmacokinetic/pharmacodynamic profiles using PEGylation. The site-specific (Lys 34 ) PEGylated GLP-1s were synthesized with PEGs of 2, 5, and 10 kDa, res