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Expression, purification and biochemical characterization of the N-terminal regions of human TIG3 and HRASLS3 proteins

✍ Scribed by Byeong-Gu Han; Jea-Won Cho; Young-Doo Cho; Soo-Youl Kim; Hye-Jin Yoon; Hyun Kyu Song; Hae-Kap Cheong; Young-Ho Jeon; Dong-ki Lee; Sangho Lee; Byung Il Lee

Book ID: 116829115
Publisher: Elsevier Science
Year: 2010
Tongue: English
Weight: 541 KB
Volume: 71
Category: Article
ISSN: 1046-5928
DOI: 10.1016/j.pep.2010.01.018

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## Abstract We have cloned, over expressed, and purified one of the two catalytic domains (residues Ala^361^ to Gly^468^, ACE‐N) of human somatic angiotensin‐I converting enzyme in __Escherichia coli.__ This construct represents the __N__‐catalytic domain including the two binding motifs and the 23