Expression profiling using cDNA microarrays

✍ Scribed by Duggan, David J; Bittner, Michael; Chen, Yidong; Meltzer, Paul; Trent, Jeffrey M.

• Book ID: 109812910
• Publisher: Nature Publishing Group
• Year: 1999
• Tongue: English
• Weight: 459 KB
• Volume: 21
• Category: Article
• ISSN: 1061-4036
• DOI: 10.1038/4434

✦ Synopsis

cDNA microarrays are capable of profiling gene expression patterns of tens of thousands of genes in a single experiment. DNA targets, in the form of 3' expressed sequence tags (ESTs), are arrayed onto glass slides (or membranes) and probed with fluorescent- or radioactively-labelled cDNAs. Here, we review technical aspects of cDNA microarrays, including the general principles, fabrication of the arrays, target labelling, image analysis and data extraction, management and mining.