The development of Cre-lox technology has created new opportunities for studying the tissue-specific functions of genes in vivo during development and disease. We analyzed the spatial and temporal activity of Cre recombinase whose coding sequence was inserted into the endogenous locus for keratin 19
Expression profiling of the mouse early embryo: Reflections and perspectives
β Scribed by Minoru S.H. Ko
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 444 KB
- Volume
- 235
- Category
- Article
- ISSN
- 1058-8388
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## Abstract DNA and RNA were extracted from mouse embryos at several stages prior to implantation. RNA was measured by extinction at 200 nm, and DNA by a fluorometric assay. The mean values of RNA in the ovulated oocyte, 1βcell, 2βcell, and blastocystβstage embryo were 0.554, 0.545, 0.396, 0.458, a