𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Expression profiling: DNA arrays in many guises

✍ Scribed by Samuel Granjeaud; François Bertucci; Bertrand R. Jordan

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
165 KB
Volume
21
Category
Article
ISSN
0265-9247

No coin nor oath required. For personal study only.

✦ Synopsis

DNA arrays have become the preferred method for large-scale expression measurement. Such data are needed in view of the large amounts of sequence data available: expression levels in a number of different tissues or situations provide a first step toward functional characterisation of new entities revealed by DNA sequencing. Although the basic principle of measurement is in all cases based on hybridisation of a mixed probe derived from tissue RNA to large sets of DNA fragments representing many genes, a number of different forms of implementation of this principle are at hand. They are briefly described and compared, emphasizing the important issue of sensitivity and sample requirements and the accessibility of the methods to academic scientists. When these factors are taken into account, it appears that, contrary to a largely prevalent impression, the ''best'' approach is not necessarily always provided by the widely advertised glass microarrays or oligonucleotide chips. BioEssays 21:781-790, 1999.

📜 SIMILAR VOLUMES

Breast cancer revisited using DNA array-
Breast cancer revisited using DNA array-based gene expression profiling
✍ François Bertucci; Patrice Viens; Pascal Hingamp; Valery Nasser; Rémi Houlgatte; 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 French ⚖ 441 KB

## Abstract Breast cancer is a complex genetic disease characterized by the accumulation of multiple molecular alterations. The resulting clinical heterogeneity makes current diagnostic and therapeutic strategies less than perfectly adapted to each patient. Pathological and clinical factors are ins

Profiling of retinoid mediated gene expr
Profiling of retinoid mediated gene expression in synchronized human SCC cells using Atlas™ human cDNA expression arrays
✍ Quan Le; Dianne Robert Soprano; Kenneth J. Soprano 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 448 KB 👁 2 views

## Abstract While retinoids have been demonstrated to inhibit growth of many tumor cells, including SCC cells, the molecular mechanism by which retinoids suppress growth has not been elucidated. We previously found that the growth of SCC cells was significantly inhibited by all‐__trans__‐retinoic a

Gene expression profiling of acute cellu
Gene expression profiling of acute cellular rejection in rat liver transplantation using DNA microarrays
✍ Naoki Hama; Yuka Yanagisawa; Keizo Dono; Shogo Kobayashi; Shigeru Marubashi; Hir 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 854 KB

Acute cellular rejection (ACR) is still a major problem in organ transplantation, and its genetic and molecular mechanisms remain poorly understood. We used DNA microarrays to investigate the gene expression profiles in ACR. We hypothesized that changes of gene expression in grafts could also be det

High density DNA array analysis reveals
High density DNA array analysis reveals distinct genomic profiles in a subset of gastrointestinal stromal tumors
✍ Martin G. Belinsky; Yuliya V. Skorobogatko; Lori Rink; Jianming Pei; Kathy Q. Ca 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 671 KB

## Abstract Gastrointestinal stromal tumors (GISTs) generally harbor activating mutations in __KIT__ or platelet‐derived growth facter receptor (__PDGFRA__). Mutations in these receptor tyrosine kinases lead to dysregulation of downstream signaling pathways that contribute to GIST pathogenesis. GIS