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Expression profiles of micro RNA in proliferating and differentiating 32D murine myeloid cells

✍ Scribed by Bin Shi; Marco Prisco; George Calin; Chang-gong Liu; Giuseppe Russo; Antonio Giordano; Renato Baserga

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
115 KB
Volume
207
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

32D cells are murine myeloid cells that grow indefinitely in Interleukin‐3 (IL‐3). In these cells, the type 1 insulin‐like growth factor (IGF‐I) and granulocytic‐colony stimulating factor (G‐CSF) induce differentiation to granulocytes. 32D cells do not express insulin receptor substrate‐1 (IRS‐1) or IRS‐2, docking proteins of the IGF‐I receptor. Ectopic expression of IRS‐1 in these cells inhibits differentiation, the cells become IL‐3 independent and IGF‐1 dependent and can form tumors in mice. 32D and 32D‐derived cells offer a good model in which to study the expression profiles of Micro Rna (miR) related to sustained proliferation or differentiation. We present here the data obtained with miR micro‐arrays and identify the miR that are regulated by IGF‐1 or G‐CSF and are associated with either differentiation or indefinite cell proliferation of 32D murine myeloid cells. © 2006 Wiley‐Liss, Inc.

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