✦ LIBER ✦

Expression patterns of collagen types I and III in the capsule of a rat knee contracture model

✍ Scribed by Yoshihiro Hagiwara; Akira Ando; Yoshito Onoda; Hiroyuki Matsui; Eiichi Chimoto; Hideaki Suda; Eiji Itoi

Publisher: Elsevier Science
Year: 2009
Tongue: English
Weight: 325 KB
Volume: 28
Category: Article
ISSN: 0736-0266
DOI: 10.1002/jor.20997

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Our objective was to determine the changes in expression of collagen types I and III in the capsule of a rat knee contracture model. The unilateral knee joints of adult male rats were rigidly immobilized at 150° of flexion using a rigid plastic plate and screws for 3 days, 1, 2, 4, 8, and 16 weeks (immobilized group). Sham‐operated animals had holes drilled in the femur and tibia with screws inserted without a plate (control group). The expression patterns of collagen types I and III in the anterior and posterior capsule were evaluated by in situ hybridization (ISH), quantitative real‐time polymerase chain reaction (qPCR), immunohistochemistry (IHC), and Western blotting (WB). Expressions of collagen types I and III were decreased after immobilization compared to the control group by ISH and qPCR. The expression was not changed after immobilization compared to the control group by IHC and WB. The expression of mRNA and protein levels of collagen types I and III were not increased after immobilization, which indicated that accumulation of the two types of collagen was not the etiology of joint contracture. Another process, such as capsule and synovial adhesions, may be one possible cause of joint contracture. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:315–321, 2010

📜 SIMILAR VOLUMES

Types I and III collagen in the tissue c

Types I and III collagen in the tissue capsules of titanium and stainless-steel implants

✍ Shannon, C. ;Thull, R. ;von Recum, A. 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 158 KB 👁 2 views

Collagen plays an important role in wound healing and as such is present in connective tissue capsules around implanted materials. The proportion of type I collagen to type III collagen is lower during wound healing than that found in normal dermis, but the amount of type I collagen gradually increa

An α1 II Gly913 to Cys substitution prev

An α1 II Gly913 to Cys substitution prevents the matrix incorporation of type II collagen which is replaced with type I and III collagens in cartilage from a patient with hypochondrogenesis

✍ Mundlos, Stefan; Chan, Danny; McGill, Jim; Bateman, John F. 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 37 KB 👁 1 views

A heterozygous mutation in the COL2A1 gene was identified in a patient with hypochondrogenesis. The mutation was a single nucleotide transition of G3285T that resulted in an amino acid substitution of Cys for Gly9I3 in the al(I1) chain of type I1 collagen. This amino acid change disrupted the obliga

Joint immobilization reduces the express

Joint immobilization reduces the expression of sensory neuropeptide receptors and impairs healing after tendon rupture in a rat model

✍ Daniel K.-I. Bring; Carol Reno; Per Renstrom; Paul Salo; David A. Hart; Paul W. 📂 Article 📅 2009 🏛 Elsevier Science 🌐 English ⚖ 203 KB 👁 1 views

## Abstract Healing after mobilization versus immobilization was assessed in a model of rat Achilles tendon rupture, by RT‐PCR at 8 and 17 days and by histological analyses at 14 and 28 days postrupture. The expression of mRNA for extracellular matrix (ECM) molecules (collagen type I and type III,