𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Expression, One-Step Purification, and Immobilization of HaloTagTMFusion Proteins on Chloroalkane-Functionalized Magnetic Beads

✍ Scribed by Hassan Motejadded; Bertolt Kranz; Sonja Berensmeier; Matthias Franzreb; Josef Altenbuchner


Book ID
107565658
Publisher
Springer-Verlag
Year
2010
Tongue
English
Weight
267 KB
Volume
162
Category
Article
ISSN
0273-2289

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


One-step purification, covalent immobili
✍ Cesar Mateo; Gloria FernΓ‘ndez-Lorente; Estrella CortΓ©s; JosΓ© L. Garcia; Roberto πŸ“‚ Article πŸ“… 2001 πŸ› John Wiley and Sons 🌐 English βš– 482 KB

## Abstract Epoxy supports covalently immobilize proteins following a two‐step mechanism; that is, the protein is physically adsorbed and then the covalent reaction takes place. This mechanism has been exploited to combine the selectivity of metal chelate affinity chromatography with the covalent i