Expression on human thymocytes of the idiotypic structures (Ti) from two leukemia T cell lines Jurkat and HPB-ALL
✍ Scribed by Stefan Carrel; Patrick Isler; Magali Schreyer; Angelo Vacca; Susanne Salvi; Laura Giuffre; Jean-Pierre Mach
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 566 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
The expression on a significant number of thymocytes of idiotypic structures (Ti) restricted to HPB-ALL or Jurkat cells is demonstrated. As many as 2-4% of thymocytes were stained with anti-Ti HPB-ALL or anti-Ti Jurkat monoclonal antibodies, when analyzed by flow microfluorometry. Immunohistochemical localization studies performed on frozen thymus specimens of either fetal or pediatric origin indicated a scattered distribution of Ti-positive cells in both the cortex and the medulla. From lysates of 125I-labeled pediatric thymocytes, anti-Ti HPB-ALL and anti-Ti Jurkat monoclonal antibodies precipitated disulfide-linked heterodimers comparable to those precipitated from 125I-labeled HPB-ALL or Jurkat cells as shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis.