Expression on a human cell line (Daudi) lacking both HLA and β2 -microglobulin of HLA-D products and cell-mediated lympholysis targets

✍ Scribed by D. Charmot; M. Kamoun; P. Crosier; M. Sasportes; M. Fellous; C. Mawas

• Publisher: John Wiley and Sons
• Year: 1976
• Tongue: English
• Weight: 424 KB
• Volume: 6
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830061215

✦ Synopsis

Abstract

The Daudi cell line which originated from a Burkitt lymphoma has recently been found lacking in HLA and β~2~‐microglobulin (β~2~ m), but carrying surface antigens, coded for by the human major histocompatibility complex, which are expressed on B cell‐enriched normal human peripheral blood lymphocytes. This report demonstrates that the stimulating property of the Daudi cell line in a mixed lymphocyte culture is indeed related to the expression of HLA‐D for which this cell line has been typed by the primed lymphocyte test; furthermore, this cell line was found to be an excellent target in the cell‐mediated lympholysis (CML) test for cytotoxic effector cells generated either against Daudi cells or against normal lymphocytes. Preliminary serological data obtained with the Daudi cell line suggest a correlation with CML data. Therefore β~2~m is probably not necessary for the expression of HLA‐D products nor is HLA a prerequisite for CML recognition and lysis.