Expression of two inward rectifier potassium channels is essential for differentiation of primitive human hematopoietic progenitor cells
✍ Scribed by Orian Shirihai; Bernard Attali; Daniel Dagan; Shoshana Merchav
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 233 KB
- Volume
- 177
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
A potassium inward rectifier (K ir ) current was previously shown by us to be induced in primitive hematopoietic progenitor cells, stimulated with the combination of interleukin-3 (IL-3) and stem cell factor (SCF). Biophysical features of whole cell currents implicated the involvement of more than one K ir channel type. Employing IL-3 / SCF stimulated human cord blood CD34 / 38 0 cells, we isolated and characterized different components of this current. Reverse transcription-polymerase chain reaction (RT-PCR) subcloning identified the expression of a strongly rectifying K ir channel (K ir 4.3) as well as a weakly rectifying K ir channel (K ir 1.1) in these cells. Inhibition of the expression of each of the channels suppressed progenitor cell generation by IL-3 and SCF-stimulated CD34 / 38 0 cells in 7-day suspension cultures. The variable expression of two essential inward rectifying potassium channels early in the course of hematopoietic progenitor cell differentiation may play a potentially important role in potassium homeostasis in these cells.