Expression of transforming growth factor-βI in rat ventral prostate and Dunning R3327 PAP prostate tumor after castration and estrogen treatment

✍ Scribed by Lindström, Pernilla; Bergh, Anders; Holm, Ingvar; Damber, Jan-Erik

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 926 KB
• Volume: 29
• Category: Article
• ISSN: 0270-4137
• DOI: 10.1002/(sici)1097-0045(199610)29:4<209::aid-pros2>3.0.co;2-6

✦ Synopsis

BACKGROUND.

In normal prostate, TGF-PI is associated to castration induced apoptosis. Combined castration and estrogen treatment, but not castration alone, induces apoptosis in the Dunning R3327 PAP adenocarcinoma. METHODS. TGF-P1 expression in rat ventral prostate (VP) and Dunning R3327 PAP tumor was studied after castration and estrogen treatment, using competitive RT-PCR, in situ hybridization and immunohistochemistry. RESULTS. TGF-Pl mRNA level was 6 times higher in the tumor than in the VP. Combined castration and estrogen treatment increased TGF-P1 mRNA levels in the tumor from day 3, while castration did not. The TGF-P1 expression was located in the epithelial cells. CONCLUSIONS. The Dunning R3327 PAP tumor contains high levels of TGF-P1, which are further increased by combined castration and estrogen treatment. However, since this increase is not apparent until day 3, TGF-PI probably does not contribute to the known induction of apoptosis in the tumor at day 1 after combined castration and estrogen treatment.