Expression of transforming growth factor-beta 1 and growth in soft agar differentiate prostate carcinoma-associated fibroblasts from normal prostate fibroblasts

✍ Scribed by Ignacio F. San Francisco; William C. DeWolf; Donna M. Peehl; Aria F. Olumi

• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: French
• Weight: 203 KB
• Volume: 112
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.20388

✦ Synopsis

Abstract

Carcinoma‐associated fibroblasts (CAF) promote tumor progression of pre‐neoplastic epithelial cells. To investigate the basis of this phenomenon, we compared the properties of fibroblasts cultured from normal human prostate (NHPF) to prostate CAF. NHPF and CAF were assayed for growth potential, cell death and proliferative capacity by measuring population doubling time, cell cycle distribution and capability to form colonies in soft agar. Resistance to genotoxic (UV radiation: 0–50 J/cm^2^) and chemotoxic (0–200 nM Taxol) agents were compared between CAF and NHPF by measuring cell viability and cell cycle analysis. Transforming growth factor β1 (TGF‐β1) immunoreactivity was assessed in non‐malignant and malignant prostatic tissue. No detectable differences were found when comparing CAF and NHPF with respect to population doubling time, cell cycle distribution and response to genotoxic and chemotoxic agents. The mean number of colonies in soft agar was 120.5 for CAF vs. 18.2 for NHPF (p < 0.05). Because TGF‐β1 and matrix metalloproteinase (MMP)‐9 have been associated with growth of fibroblasts in soft agar and tumor promotion, we measured the expression of these factors in NHPF and CAF by ELISA. There was no difference in expression of MMP‐9; however, TGF‐β1 was expressed in higher concentrations in CAF than in NHPF (p < 0.0014). Furthermore, TGF‐β1 expression was higher in the carcinoma‐associated stroma of prostate cancer tissue than stroma of non‐malignant prostatic tissue. Increased capability of CAF as compared to NHPF to form colonies in soft agar may be due to a higher expression of TGF‐β1 and correlates with the ability of CAF to promote malignant progression of prostate epithelial cells. © 2004 Wiley‐Liss, Inc.