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Expression of the xylan-degrading genes of Bacillus pumilus IPO in Saccharomyces cerevisiae

✍ Scribed by Cai-Xi Pan; Wei-Zhong Xu; Hiroyuki Akatsuka; Seiji Negoro; Yasufumi Shima; Itaru Urabe; Hirosuke Okada


Book ID
113396691
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
586 KB
Volume
71
Category
Article
ISSN
0922-338X

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Molecular cloning of the genes for xylan
✍ Panbangred, Watanalai ;Kondo, Tetsuya ;Negoro, Seiji ;Shinmyo, Atsuhiko ;Okada, πŸ“‚ Article πŸ“… 1983 πŸ› Springer 🌐 English βš– 767 KB

The 7.7 Mdal PstI fragment of Bacillus pumilus IPO containing genes for xylan degradation, xylanase, and fl-xylosidase was inserted at the Pstl site of pBR322 and cloned in E. coli C600. The hybrid plasmid thus formed was named pOXN29. The amount of xylanase and fl-xylosidase expressed in E. coli ha