Expression, of the thrombospondin receptor (CD36) on the cell surface in megakaryoblastic and promegakaryocytic leukemias: Increment of the receptor by megakaryocyte differentiation in vitro

✍ Scribed by Dr. Nobutaka Imamura; Hiromi Ota; Kazuhiro Abe; Atsushi Kuramoto

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 308 KB
• Volume: 45
• Category: Article
• ISSN: 0361-8609
• DOI: 10.1002/ajh.2830450216

✦ Synopsis

In this report we describe four cases of acute megakaryocytic leukemia demonstrated by the presence of megakaryocyteplatelet-related cell-surface antigens. These were detected utilizing flow cytometry and monoclonal antibodies In addition to both platelet peroxldase activity, which was shown by ultrastractural cytochemistry, and emergence of differentiation antigens, while culturing these leukemic cells. The blasts of one patient possessed both platelet Gplb and Gpllb/llla cell-surface antigens detected by AN51(CD42b), J15, P2, and HPL2(CD41), respectively, whereas the remainlng three patients almost completely lacked Gplb cell-surface antigen. Hence the former were diagnosed as immature(pro)megakaryocylic leukemla and the latter as acute megakaryoblastic leukemia from the viewpoint of immunophenotyplc analysis. While we cultured these leukemic cells In conditioned medlum prepared from phytohemagglutinln-stimulated leukocytes and interleukln 3, expression of CD36(OKM5) antigen (thrombospondin receptor) increased gradually according to the differentiation and maturation of these cells. Finally, all leukemic cells differentiated to mature megakaryocytes. The function of CD36 on these cells remains to be elucidated.