Expression of the PreS1 Peptide of Hepatitis B Virus and Preparation of Its Polyclonal Antibody
โ Scribed by Chen, Weixian ;Zhang, Jun ;Zhang, Juan ;Wang, Ding ;Li, Yue ;Huang, Ailong
- Book ID
- 117991561
- Publisher
- Mary Ann Liebert
- Year
- 2011
- Tongue
- English
- Weight
- 157 KB
- Volume
- 30
- Category
- Article
- ISSN
- 1554-0014
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The diagnostical significance of the large hepatitis B surface protein with its preS 1 attachment site and of anti-preS antibodies are not yet well known. We investigated the epitope of the preS1 attachment site to see whether it is a marker of viremia and whether antibodies against it occur in conv
## Abstract The infectious particles of hepatitis B virus (HBV) contain 3 related surface antigens, i.e., small, medium, and large, all of which are encoded by one large open reading frame with multiple initiation codons. The large surface antigen (LโAg) contains preS1, preS2, and S regions while b
The complete (encoding 119 aminon acids, aa) or partial (encoding the N-terminal 90 aa) preS1 region gene of hepatitis B virus (HBV) was fused to the 3'-end of glutathione-S-transferase (GST) gene and expressed at 37 ยฐC under the control of the inducible tac promoter in E. coli. The results showed t